HBsAg ELISA KIT

Anti-HIV1+2 is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of antibodies to human immunodeficiency virus type 1 and/or 2 in human serum or plasma.

DAAN HBsAg ELISA is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of Hepatitis B Surface Antigen(HBsAg) in human serum or plasma.

Sensitive enzyme immunoassays for the detection of HBsAg were first described by Engvall and Perlmann and VanWeemen and Schuurs in 1971. In 1976 and 1977, solid phase "sandwich" enzyme immunoassays were developed in which HBsAg was captured on a solid phase coated with polyclonal antibodies against HBsAg (anti-HBs) and then detected with anti-HBs conjugated to an enzyme. In recent years, monoclonal anti-HBs assays have been developed for the detection of HBsAg.

The wells of the polystyrene microplate strips are coated with mouse monoclonal anti-HBs. Both human serum or plasma and polyclonal anti-HBs labeled with horseradish peroxidase are incubated in these coated wells. HBsAg, if present, will bind to the solid phase antibody and the labeled antibody resulting in the HBsAg being sandwiched between the solid phase antibody and labeled antibody. Excess unbound HBsAg and labeled antibodies are removed by washing. Substrate solution containing hydrogen peroxide and 3,3',5,5'-tetramethyl- benzidine(TMB) is then added to each well. The presence of HBsAg is indicated by the presence of a blue color after substrate addition. Reaction is terminated by addition of sulphuric acid. The intensity of the color is measured spectrophotometrically at 450nm and is proportional to the amount of HBsAg present in the specimen.

Contains stabilizing proteins and detergent, Preservative: Bronidox (2ml/L).