cccDNA (covalently closed circular DNA) is covalently closed circular DNA, which
is the form in which almost all plasmid DNA and some viral DNA (e.g., HBV cccDNA) is present, and is often found
It is also found in disease cells of some higher organisms. Its cccDNA in human and other eukaryotic cells is also calledeccDNA (extrachromosomal circular DNA), which are usually isolated from the normal genome or
are usually isolated or shed from the normal genome and are free from the chromosomal genome.
The DNA is usually isolated or shed from the normal genome. Whole-genome sequence analysis of eccDNA from yeast, nematodes, ciliates, and mammals revealed that the size of eccDNA ranged from
The size of eccDNA varies from a few tens to hundreds of thousands of base pairs and carries not only complete or partial
The eccDNAs range in size from a few tens of bases to hundreds of thousands of base pairs and carry not only complete or partial genes but also intergenic sequences, and some of them contain replication initiation sites and can complete replication independently.
eccDNA includes small polydispersed circular DNA (spcDNA) and telomeric
circles (t-circles). Therefore, the isolation and purification of these DNAs are important for subsequent studies. For this reason
We have developed a kit for the purification of cccDNA, which has the following features: 1.
1. ready to use, the user does not have to develop their own special kits. 2.
2. It can effectively separate cccDNA and non-cccDNA. 3.
3. The product is based on precipitation method, recovering cccDNA with high efficiency. 4.
4. Compatible with subsequent enzymatic electrophoresis and nucleic amplification studies. 5.
5. This kit is sufficient for 50 cccDNA purification experiments. 6.
6. This product can only be used for research, not for clinical use.
