CAS.NO: 9017-74-8
Recombinant Enterokinase is a kind of highly purified recombinant bovine enterokinase light chain. The enzyme has been extensively purified by HPLC and there are no traces of other contaminating proteases.
Recombinant Enterokinase Specifications
Product Name | Recombinant Enterokinase |
Source | Recombinant E. coli |
Appearance | Colorless to light yellow clear liquid |
Activity | ≥5.0 U/μl |
Purity(SDS-PAGE) | Single major band 25.8±2.6kDa |
Storage Conditions | -15°C below,protected from light, sealed. |
Recombinant Enterokinase Application
Enzyme digestion condition: 25mM Tris-HCl 8.0, fusion protein concentration: 0.1-1mg/ml (total protein content: 50-100μg), EK content: 0.1-2U, Temperature: 25℃, overnight or 16h-24h for digestion.
4°C low temperature digestion conditions: 4°C can effectively digest the substrate, but need to extend the digestion time to 48-64h, or increase the enzyme 2-3 times.
Enzyme cleavage optimization and amplification: Optimization: Enzyme cleavage conditions can be optimized, such as buffer pH, fusion protein concentration, EK amount, and digestion time, so that the fusion protein can be digested under stable conditions, SDS-PAGE detection of digestion effect. Amplification: Select the optimized reaction conditions, according to the conditions such as the proportion of amplification, SDS- PAGE detection of digestion effect.
＞200mM imidazole or ＞200mM NaCl or ＞5% glycerin, the reaction may be effected.The following suggestions are given: To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0. If the dialysis is inconvenient, please dilute the sample to ＜ 100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:500μg fusion protein). If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.
Enterokinase Enzyme Transport and Storage Stability
Stability of storage: Recombinant enterokinase and enteropeptidase should be stored at -15°C below in sealed container.
It is stable within 24 months.If stored at 25°C ,it is stable within one week.
It is no activity loss after 5 times repeated freezing and thawing.
Stability of transport: The product is stable by ice insulation transport.
Notice and Disclaimer
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
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