Progesterone (P4) is a kind of steroid hormone. The concentration change of P4 in milk is one specific characteristic indicating the activities of cow ovary. It plays an important role in the process of maternal reproduction and maintaining pregnancy.
Therefore, it can be monitoring cow reproductive activity by detecting the concentration of P4. P4 concentration has different characteristic changes as the cow's stage of reproductive physiology, through to detect the progesterone levels can make an accurate diagnosis of cow in early pregnancy.
SPECIMEN COLLECTION
1.Milk sample: cleaning teats before take the milk, abandon to the first three milk, then collect milk in the bottle, take 1 ml in the test tube, placed the centrifuge10000 rpm for 10 minutes, in vitro milk sample was divided into three layers, using straws inserted in the underlying absorbing liquid cream and set aside.
2.Saliva: from the mouth collecting saliva for 3 to 5 ml placed the centrifuge 1500-2000 rpm for 5 minutes, take supernatant on standby.
3.The galley proof: from the hair root galley proof according to take 10 mg in the extracting pipe, add 3 ml anhydrous ethanol soak vortex shock 5 minutes after 2-3 hours, placed 3000 rpm centrifuge for 5 minutes, drain on ethanol solution 1 ml into glass dish, in 70-80 ℃ oven dry reoccupy after 1 ml PBS dilution. Set aside.
4.Urine: can be used directly.
5.Blood: anticoagulant take 1 to 2 ml of centrifugal 2000 rpm 5-10 minutes from the plasma standby, the anticoagulant let stand for 30 minutes to separate serum blood clots. Set aside.
