Product name: Fluorescent phycocyanin
Molecular Weight:38000
Ratio (A620/A280): >=4.5
Maximum absorbance:620+_5nm
Fluorescence emission peak:640+-5nm
Stability:Can stored in ammonium sulphate solution (45%) for one year
Appearance: blue or dark blue powder.
Product Description:
Fluorescent phycocyanin, which has a unique optical property of new fluorescent markers, is isolated and purified from Spirulina.FPC emit strong fluorescence, with good performance and high absorption of the quantum productivity, and the intensity of fluorescein is 30 times stronger than the commonly. It is used in the visible spectrum over a wide range for stimulating and firing.
The preparation process of strong fluorescent phycocyanin using fresh smashed algae as raw material includes: washing with EDTA containing phosphoric acid buffering solution, ultrasonic crushing of cell to release pigment protein, centrifugal separation to eliminate insoluble cell membrane and organelle and to collect the supernatant; salting out phycocyanin precipitant; dissolving the precipitant in EDTA containing phosphoric acid buffering solution, desalting with Sephadex G-25, eliminating alkali impurity protein in DEAE-Sepharose FF column; obtaining phycocyanin eluent in hydroxyapatite column; eliminating acid impurity protein in CM-Sepharose FF column; and finally obtaining high-purity strong fluorescent phycocyanin in hydroxyapatite column. The process is suitable for large-scale industrial production.
