Recombinant Enterokinase or recombinant bovine enterokinase EC 3.4.21.9
Recombinant Enterokinase / Enterokinase / REK / High specificity / High purity
DESCRIPTION
Recombinant Enterokinase
YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been
extensively purified and there are no traces of other contaminating proteases. Enterokinase is a specific
protease that cleaves lysineC-terminal preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. However, enterokinase does not work if this lysine is followed by a proline. Enterokinase can
remove N-terminal fusion protein,it is very useful for removing unwanted protein tags.
ADVANTAGES
High purity
(Figure 1)
(Figure 2)
Figure 1: One single main band by SDS-PAGE analysis
Figure 2: SDS-PAGE analysis of 0. 1 U EK effect on 50µg substrate after 0min, 10min, 20min, 30min, 40min
50min and 60min, respectively
High specificity
1. Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)
2. No any other contaminated proteases, no non-specific cutting sites.
Conditions
Cutting condition: given an example: 25mM Tris-HCl 8.0
Fusion protein concentration: 0.1-1mg/ml (total protein content: 0.5-1.0mg)
EK content: 1-2U
Temperature: 25
Time: overnight or 12h-16h for digestion.
Common components influence the action of enterokinase
200mM imidazole or 200mM NaCl or 5%glycerin, the reaction may be effected.The following
suggestions are given
1. To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.
2. If the dialysis is inconvenient, pleasedilute the sample to 100mM imidazole, 50mMNaCl, 5%
glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).
3. If there are one or more components in samples, and cannot be removed, suggest to increase the
content of EK in reaction system or extend the reaction time.
MAIN FEATURES
|
Source |
E.Coli |
|
M.W. |
Theoretical MW: 25,850 Da;The apparent MW on SDS-PAGE: about 27,000 Da |
|
Specific Activity |
One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in a buffer 25mMTris-HCl, pH 8.0.Substrate: a special fusion protein. |
|
Storage |
Store at -20°C after delivery. |
|
Stability |
Keep cool with blue ice during shipping.Remained stable at 25°C for one week without activity lost. Can be stable after several times of freeze-thaw. |
PRODUCT INFORMATION
|
Product |
Cat.No |
Specific Activity |
Packaging |
Manufacturer |
|
Recombinant Enterokinase |
EK0801 |
≥1 u/μl;≥5 u/μl |
100U, 1KU or bulk |
YaxinBio |
