recombinant enzymes Aspartate protease for Asp-C / Glu-c sequencing grade
V8 / Endoproteinase Glu-C / V8 Protease / serine proteinase
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FULL NAME |
Endoproteinase Glu-C,from Staphylococcus aureus strain V8,V8 Protease
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CAS NUMBER |
66676-43-5
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EC NUMBER |
3.4.21.19
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DESCRIPTION
Staphylococcus Aureus v8 Protease
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves
peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The Optimum
pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2
- macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
SPECIFIC ACTIITY
Approx.20U/mg ar 25 with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37 with casein as the substrate).
INSTRUCTIONS FOR USE
Cutting condition:PH 8.0~8.5
PACKAGING
1U/vial,20 mg/vial
STORAGE
-20
APPLICATION
Protein structure and sequence analysis
RELATED PRODUCT
Recombinant Trypsin(sequencing purity);
Recombinant Carboxypeptidase B(sequencing purity);
Recombinant Chymotrypsin(sequencing purity);
V8(sequencing purity);
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