CAS 9004-07-3 / recombinant chymotrypsin for Enzymatic Hydrolysis of Protein
Recombinant Chymotrypsin / Chymotrypsin / RCT / rCT
DESCRIPTION
Chymotrypsin Enzyme
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene
sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the
carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower
rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing grade
enzyme can be used alone or in combination with other proteases to produce protein digests for peptide
mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.
It is suitable for digestion reactions in-solution or in-gel.
RELATED PRODUCT
Recombinant Porcine Trypsin
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