Chymotrypsin proteomics grade / chymotrypsin for peptide mass fingerprinting
Sequencing Grade Chymotrypsin / Chymotrypsin / SRCT / sequencing grade enzyme
DESCRIPTION
Sequencing Grade Trypsin
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene
sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl
side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at
Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0.
MAIN FEATURES
1. Source E. Coli
2. Purified by HPLC
3. Physical form Lyophilized powder
4. Specific activity NLT 1500 USP units/mg pro
5. Contaminant activity No carboxypeptidase A, or other proteases contaminant.
APPLICATION
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or
MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
RECOMMEND USAGE
To prepare 1-10mg/ml with 1ml 1mM HCl, used within 2 days, or stored below -20 after repacked
RELATED PRODUCT
Recombinant Trypsin (sequencing purity);
Recombinant Carboxypeptidase B (sequencing purity);
Recombinant Chymotrypsin (sequencing purity);
V8 (sequencing purity).
Web:http://www.yaxinbio.com Email:Zhaozhi@yaxinbio.com
