| CAS: | 108321-27-3 |
| MF: | C8H19KN2O6S2 |
| MW: | 342.47 |
| storage temp. | Store at RT. |
| solubility | H2O: 0.5 g/mL, clear, colorless |
| pka | 6.8(at 25℃) |
| PH Range | 6.1 - 7.5 |
| CAS DataBase Reference | 108321-27-3(CAS DataBase Reference) |
General description
PIPES (1,4-Piperazinediethanesulfonic ) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.
Application
PIPES (1,4-Piperazinediethanesulfonic ) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.
